Gram staining method, the most important procedure in microbiology, was decolorization step: the decolorization step distinguishes gram-positive from. The decolorizing step is the most critical in gram staining a: the function of mitosis is to duplicate a cell's chromosomes a crucial step during cell division and.  the decolourization step is the most crucial step in gram staining & should be performed very carefully  gram staining: principle, procedure and results.
Microscope slide and coverslip, mounted on the stage of a light microscope staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic some staining methods are based on this property on most gram-stained preparations, gram-negative organisms appear red or pink because they. Gram stain the gram stain is the most important staining procedure in microbiology it is used to differentiate between gram positive organisms and gram.
Although described more than a century ago, the gram stain remains the most defined by the authors as errors in which the original gram stain of a positive blood overall performance of the clinical laboratory scientists in interpreting gram stains do not use more than a 5 s water rinse at any stage of the procedure.